Rafał Tomecki, PhD, DSc
Postdoc
(+48) 22 592 2034
Room no. 034
Involved in: RNA Biology
Academic and Research Career:
Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw;
Assistant Professor, since October 2011
Institute of Biochemistry and Biophysics, Polish Academy of Sciences;
Assistant Professor, since October 2008
Institute of Biochemistry and Biophysics, Polish Academy of Sciences;
Research Associate; 2008
Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw;
Senior Research Associate; 2007-2010
Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw;
Research Associate; 2007
Department of Biology, Technion – Israel Institute of Technology; Haifa, Israel;
Visiting Graduate Student; 2006
Department of Neurology, University of Newcastle upon Tyne; Great Britain;
Visiting Graduate Student; 2004
Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw;
PhD Student; 2002-2007
Faculty of Biology, University of Warsaw;
MSc Student; 1997-2002
Publications:
Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target.
Tomecki R, Drazkowska K, Kucinski I, Stodus K, Szczesny RJ, Gruchota J, Owczarek EP, Kalisiak K, Dziembowski A.
Nucleic Acids Res. 2013 Oct 22. [Epub ahead of print]
Exonuclease hDIS3L2 specifies an exosome-independent 3′-5′ degradation pathway of human cytoplasmic mRNA.
Lubas M, Damgaard CK, Tomecki R, Cysewski D, Jensen TH, Dziembowski A.
EMBO J. 2013 Jul 3;32(13):1855-68. doi: 10.1038/emboj.2013.135. Epub 2013 Jun 11.
The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro.
Drazkowska K, Tomecki R, Stodus K, Kowalska K, Czarnocki-Cieciura M, Dziembowski A.
Nucleic Acids Res. 2013 Apr 1;41(6):3845-58. doi: 10.1093/nar/gkt060. Epub 2013 Feb 12.
Catalytic properties of the eukaryotic exosome.
Chlebowski A, Tomecki R, López ME, Séraphin B, Dziembowski A.
Adv Exp Med Biol. 2011;702:63-78. doi: 10.1007/978-1-4419-7841-7_6.
The eukaryotic RNA exosome: same scaffold but variable catalytic subunits.
Lykke-Andersen S, Tomecki R, Jensen TH, Dziembowski A.
RNA Biol. 2011 Jan-Feb;8(1):61-6. Epub 2011 Jan 1. Review.
RNA channelling by the eukaryotic exosome.
Malet H, Topf M, Clare DK, Ebert J, Bonneau F, Basquin J, Drazkowska K, Tomecki R, Dziembowski A, Conti E, Saibil HR, Lorentzen E.
EMBO Rep. 2010 Dec;11(12):936-42. doi: 10.1038/embor.2010.164. Epub 2010 Nov 12.
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
Tomecki R, Dziembowski A.
RNA. 2010 Sep;16(9):1692-724. doi: 10.1261/rna.2237610. Epub 2010 Jul 30. Review.
The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.
Tomecki R, Kristiansen MS, Lykke-Andersen S, Chlebowski A, Larsen KM, Szczesny RJ, Drazkowska K, Pastula A, Andersen JS, Stepien PP, Dziembowski A, Jensen TH.
EMBO J. 2010 Jul 21;29(14):2342-57. doi: 10.1038/emboj.2010.121. Epub 2010 Jun 8.
Mechanisms of RNA degradation by the eukaryotic exosome.
Tomecki R, Drazkowska K, Dziembowski A.
Chembiochem. 2010 May 3;11(7):938-45. doi: 10.1002/cbic.201000025. Review. No abstract available.
Endonucleolytic RNA cleavage by a eukaryotic exosome.
Lebreton A, Tomecki R, Dziembowski A, Séraphin B.
Nature. 2008 Dec 18;456(7224):993-6. doi: 10.1038/nature07480. Epub 2008 Dec 7.
Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family.
Lorentzen E, Basquin J, Tomecki R, Dziembowski A, Conti E.
Mol Cell. 2008 Mar 28;29(6):717-28. doi: 10.1016/j.molcel.2008.02.018.
Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.
Gewartowski K, Tomecki R, Muchowski L, Dmochow Ska A, Dzwonek A, Malecki M, Skurzak H, Ostrowski J, Stepien PP.
Acta Biochim Pol. 2006;53(1):179-88. Epub 2006 Feb 26.
Differential stability of mitochondrial mRNA in HeLa cells.
Piechota J, Tomecki R, Gewartowski K, Szczesny R, Dmochowska A, Kudła M, Dybczyńska L, Stepien PP, Bartnik E.
Acta Biochim Pol. 2006;53(1):157-68. Epub 2005 Dec 21.
Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.
Tomecki R, Dmochowska A, Gewartowski K, Dziembowski A, Stepien PP.
Nucleic Acids Res. 2004 Nov 16;32(20):6001-14. Print 2004.
RNA degradation in yeast and human mitochondria.
Piwowarski J, Dziembowski A, Dmochowska A, Minczuk M, Tomecki R, Gewartowski K, Stepien PP.
Toxicol Mech Methods. 2004;14(1-2):53-7. doi: 10.1080/15376520490257473.
Specific induction and carbon/nitrogen repression of arginine catabolism gene of Aspergillus nidulans–functional in vivo analysis of the otaA promoter.
Dzikowska A, Kacprzak M, Tomecki R, Koper M, Scazzocchio C, Weglenski P.
Fungal Genet Biol. 2003 Mar;38(2):175-86.
Awards:
November 2011 – Team prize of the Rector of the University of Warsaw for scientific achievements
October 2011 – Scholarship of the Minister of Science and Higher Education for Outstanding Young Scientists
June 2011 – Award of the IBB PAS Research Council for the best review published in the year 2010
June 2011 – Award of the IBB PAS Research Council for the best original publication in the year 2010
June 2010 – Travel fellowship from the Warsaw Scientific Society/Foundation for Polish Science to cover the costs of participation in 12th IUBMB and 21th FAOBMB Conference: “The molecules of life – from discovery to biotechnology”
April 2010 – FEBS Scholarship to cover the costs of participation in FEBS Young Scientist Forum “Life of Molecules” oraz 35th FEBS Congress “Molecules of Life”
June 2009 – Award of the IBB PAS Research Council for the scientific activity in the year 2008
May 2009 – Postdoctoral fellowship from the Foundation for Polish Science within the “TEAM” Programme
March 2009 – IUBMB grant for participation in the Young Scientists Program during 21st IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
November 2008 – Team prize of the Rector of the University of Warsaw for scientific achievements
June 2008 – Travel fellowship from the Warsaw Scientific Society/Foundation for Polish Science to cover the costs of participation in the 13th Annual Meeting of the RNA Society
November 2007 – Team prize of the Rector of the University of Warsaw for scientific achievements
2007, 2008 – Stipends for Young Scientists from the Foundation for Polish Science within the “START” programme
2006 – FEBS Collaborative Scholarship for Central and Eastern Europe
October 2005 – Polish Biochemical Society award (J. K. Parnas prize) and the Polish Society of Genetics award for the co-authorship of the best experimental paper in Poland in year 2004
2004 – FEBS Collaborative Scholarship for Central and Eastern Europe
October 2002 – PhD Scholarship of the Postgraduate School of Molecular Medicine affiliated with the Medial University of Warsaw
June 2001 – Scholarship of the Polish Ministry of Education for outstanding examination grades and scientific achievements