Andrzej Dziembowski, Prof.

PI

(+48) 22 592 2033
Room no. 032

 

Academic and Research Career:

Institute of Biochemistry and Biophysics Polish Academy of Sciences, since 2009
Associate Professor

Institute of Genetics and Biotechnology, University of Warsaw, since 2009
Associate Professor

Institute of Genetics and Biotechnology, University of Warsaw, since 2006-2009
Assistant Professor

Centre de Génétique Moléculaire – CNRS, Gif-sur-Yvette, Paris, France 2002-2006
Post-doc, advised by dr Bertrand Seraphin

Institute of Genetics and Biotechnology, University of Warsaw, 1998-2002
PhD Student, advised by Prof, Piotr Stępień

Inter-Faculty Individual Studies in Mathematics and Natural Sciences, University of Warsaw, 1993-1997

Publications:

Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target.
Tomecki R, Drazkowska K, Kucinski I, Stodus K, Szczesny RJ, Gruchota J, Owczarek EP, Kalisiak K, Dziembowski A.
Nucleic Acids Res. 2013 Oct 22. [Epub ahead of print]

A new strategy for gene targeting and functional proteomics using the DT40 cell line.
Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS,Dziembowski A.
Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. Epub 2013 Jul 27.

U6 RNA biogenesis and disease association.
Mroczek S, Dziembowski A.
Wiley Interdiscip Rev RNA. 2013 Sep-Oct;4(5):581-92. doi: 10.1002/wrna.1181. Epub 2013 Jun 14.

Exonuclease hDIS3L2 specifies an exosome-independent 3′-5′ degradation pathway of human cytoplasmic mRNA.
Lubas M, Damgaard CK, Tomecki R, Cysewski D, Jensen TH,Dziembowski A.
EMBO J. 2013 Jul 3;32(13):1855-68. doi: 10.1038/emboj.2013.135. Epub 2013 Jun 11.

The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro.
Drazkowska K, Tomecki R, Stodus K, Kowalska K, Czarnocki-Cieciura M,Dziembowski A.
Nucleic Acids Res. 2013 Apr 1;41(6):3845-58. doi: 10.1093/nar/gkt060. Epub 2013 Feb 12.

Identification of a novel human mitochondrial endo-/exonuclease Ddk1/c20orf72 necessary for maintenance of proper 7S DNA levels.
Szczesny RJ, Hejnowicz MS, Steczkiewicz K, Muszewska A, Borowski LS, Ginalski K, Dziembowski A.
Nucleic Acids Res. 2013 Mar 1;41(5):3144-61. doi: 10.1093/nar/gkt029. Epub 2013 Jan 28.

RNA decay machines: the exosome.
Chlebowski A, Lubas M, Jensen TH, Dziembowski A.
Biochim Biophys Acta. 2013 Jun-Jul;1829(6-7):552-60. doi: 10.1016/j.bbagrm.2013.01.006. Epub 2013 Jan 23. Review.

Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci.
Borowski LS, Dziembowski A, Hejnowicz MS, Stepien PP, Szczesny RJ.
Nucleic Acids Res. 2013 Jan;41(2):1223-40. doi: 10.1093/nar/gks1130. Epub 2012 Dec 5.

The yeast THO complex forms a 5-subunit assembly that directly interacts with active chromatin.
Gewartowski K, Cuéllar J, Dziembowski A, Valpuesta JM.
Bioarchitecture. 2012 Jul 1;2(4). [Epub ahead of print]

C16orf57, a gene mutated in poikiloderma with neutropenia, encodes a putative phosphodiesterase responsible for the U6 snRNA 3′ end modification.
Mroczek S, Krwawicz J, Kutner J, Lazniewski M, Kuciński I, Ginalski K,Dziembowski A.
Genes Dev. 2012 Sep 1;26(17):1911-25. doi: 10.1101/gad.193169.112. Epub 2012 Aug 16.

Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor.
Peña A, Gewartowski K, Mroczek S, Cuéllar J, Szykowska A, Prokop A, Czarnocki-Cieciura M, Piwowarski J, Tous C, Aguilera A, Carrascosa JL, Valpuesta JM, Dziembowski A.
EMBO J. 2012 Mar 21;31(6):1605-16. doi: 10.1038/emboj.2012.10. Epub 2012 Feb 7.

Comprehensive structural and substrate specificity classification of the Saccharomyces cerevisiae methyltransferome.
Wlodarski T, Kutner J, Towpik J, Knizewski L, Rychlewski L, Kudlicki A, Rowicka M, Dziembowski A, Ginalski K.
PLoS One. 2011;6(8):e23168. doi: 10.1371/journal.pone.0023168. Epub 2011 Aug 9.

Interaction profiling identifies the human nuclear exosome targeting complex.
Lubas M, Christensen MS, Kristiansen MS, Domanski M, Falkenby LG, Lykke-Andersen S, Andersen JS, Dziembowski A, Jensen TH.
Mol Cell. 2011 Aug 19;43(4):624-37. doi: 10.1016/j.molcel.2011.06.028.

Catalytic properties of the eukaryotic exosome.
Chlebowski A, Tomecki R, López ME, Séraphin B, Dziembowski A.
Adv Exp Med Biol. 2011;702:63-78. doi: 10.1007/978-1-4419-7841-7_6.

Strategies for the structural analysis of multi-protein complexes: lessons from the 3D-Repertoire project.
Collinet B, Friberg A, Brooks MA, van den Elzen T, Henriot V, Dziembowski A, Graille M, Durand D, Leulliot N, Saint André C, Lazar N, Sattler M, Séraphin B, van Tilbeurgh H.
J Struct Biol. 2011 Aug;175(2):147-58. doi: 10.1016/j.jsb.2011.03.018. Epub 2011 Apr 2.

The eukaryotic RNA exosome: same scaffold but variable catalytic subunits.
Lykke-Andersen S, Tomecki R, Jensen TH, Dziembowski A.
RNA Biol. 2011 Jan-Feb;8(1):61-6. Epub 2011 Jan 1. Review.

RNA channelling by the eukaryotic exosome.
Malet H, Topf M, Clare DK, Ebert J, Bonneau F, Basquin J, Drazkowska K, Tomecki R, Dziembowski A, Conti E, Saibil HR, Lorentzen E.
EMBO Rep. 2010 Dec;11(12):936-42. doi: 10.1038/embor.2010.164. Epub 2010 Nov 12.

Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.
Brooks MA, Gewartowski K, Mitsiki E, Létoquart J, Pache RA, Billier Y, Bertero M, Corréa M, Czarnocki-Cieciura M, Dadlez M, Henriot V, Lazar N, Delbos L, Lebert D, Piwowarski J, Rochaix P, Böttcher B, Serrano L, Séraphin B, van Tilbeurgh H, Aloy P, Perrakis A, Dziembowski A.
Structure. 2010 Sep 8;18(9):1075-82. doi: 10.1016/j.str.2010.08.001.

Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.
Tomecki R, Dziembowski A.
RNA. 2010 Sep;16(9):1692-724. doi: 10.1261/rna.2237610. Epub 2010 Jul 30. Review.

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.
Tomecki R, Kristiansen MS, Lykke-Andersen S, Chlebowski A, Larsen KM, Szczesny RJ, Drazkowska K, Pastula A, Andersen JS, Stepien PP,Dziembowski A, Jensen TH.
EMBO J. 2010 Jul 21;29(14):2342-57. doi: 10.1038/emboj.2010.121. Epub 2010 Jun 8.

Mechanisms of RNA degradation by the eukaryotic exosome.
Tomecki R, Drazkowska K, Dziembowski A.
Chembiochem. 2010 May 3;11(7):938-45. doi: 10.1002/cbic.201000025. Review. No abstract available.

Catalytic properties of the eukaryotic exosome.
Chlebowski A, Tomecki R, López ME, Séraphin B, Dziembowski A.
Adv Exp Med Biol. 2010;702:63-78. Review.

Endonucleolytic RNA cleavage by a eukaryotic exosome.
Lebreton A, Tomecki R, Dziembowski A, Séraphin B.
Nature. 2008 Dec 18;456(7224):993-6. doi: 10.1038/nature07480. Epub 2008 Dec 7.

Structure of the yeast Pml1 splicing factor and its integration into the RES complex.
Brooks MA, Dziembowski A, Quevillon-Cheruel S, Henriot V, Faux C, van Tilbeurgh H, Séraphin B.
Nucleic Acids Res. 2009 Jan;37(1):129-43. doi: 10.1093/nar/gkn894. Epub 2008 Nov 25.

Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family.
Lorentzen E, Basquin J, Tomecki R, Dziembowski A, Conti E.
Mol Cell. 2008 Mar 28;29(6):717-28. doi: 10.1016/j.molcel.2008.02.018.

RNA channelling by the archaeal exosome.
Lorentzen E, Dziembowski A, Lindner D, Seraphin B, Conti E.
EMBO Rep. 2007 May;8(5):470-6. Epub 2007 Mar 23.

A single subunit, Dis3, is essentially responsible for yeast exosome core activity.
Dziembowski A, Lorentzen E, Conti E, Séraphin B.
Nat Struct Mol Biol. 2007 Jan;14(1):15-22. Epub 2006 Dec 17.

Subunit architecture of multimeric complexes isolated directly from cells.
Hernández H, Dziembowski A, Taverner T, Séraphin B, Robinson CV.
EMBO Rep. 2006 Jun;7(6):605-10. Epub 2006 May 19.

Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing.
Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprévote O, Séraphin B.
EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25.

Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase.
Tomecki R, Dmochowska A, Gewartowski K, Dziembowski A, Stepien PP.
Nucleic Acids Res. 2004 Nov 16;32(20):6001-14. Print 2004.

Recent developments in the analysis of protein complexes.
Dziembowski A, Séraphin B.
FEBS Lett. 2004 Jan 2;556(1-3):1-6. Review.

RNA degradation in yeast and human mitochondria.
Piwowarski J, Dziembowski A, Dmochowska A, Minczuk M, Tomecki R, Gewartowski K, Stepien PP.
Toxicol Mech Methods. 2004;14(1-2):53-7. doi: 10.1080/15376520490257473.

Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria.
Piwowarski J, Grzechnik P, Dziembowski A, Dmochowska A, Minczuk M, Stepien PP.
J Mol Biol. 2003 Jun 20;329(5):853-7.

Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA.
Minczuk M, Piwowarski J, Papworth MA, Awiszus K, Schalinski S,Dziembowski A, Dmochowska A, Bartnik E, Tokatlidis K, Stepien PP, Borowski P.
Nucleic Acids Res. 2002 Dec 1;30(23):5074-86.

The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism.
Dziembowski A, Piwowarski J, Hoser R, Minczuk M, Dmochowska A, Siep M, van der Spek H, Grivell L, Stepien PP.
J Biol Chem. 2003 Jan 17;278(3):1603-11. Epub 2002 Nov 7.

Genetic and biochemical approaches for analysis of mitochondrial degradosome from Saccharomyces cerevisiae.
Dziembowski A, Stepien PP.
Methods Enzymol. 2001;342:367-78. No abstract available.

Analysis of 3′ and 5′ ends of RNA by solid-phase S1 nuclease mapping.
Dziembowski A, Stepien PP.
Anal Biochem. 2001 Jul 1;294(1):87-9. No abstract available.

Yeast nuclear PET127 gene can suppress deletions of the SUV3 or DSS1 genes: an indication of a functional interaction between 3′ and 5′ ends of mitochondrial mRNAs.
Wegierski T, Dmochowska A, Jabłonowska A, Dziembowski A, Bartnik E, Stepień PP.
Acta Biochim Pol. 1998;45(4):935-40.

The yeast nuclear gene DSS1, which codes for a putative RNase II, is necessary for the function of the mitochondrial degradosome in processing and turnover of RNA.
Dziembowski A, Malewicz M, Minczuk M, Golik P, Dmochowska A, Stepien PP.
Mol Gen Genet. 1998 Oct;260(1):108-14.

Selected Professional Merits and Awards:

2001. Scholarship from the Foundation for Polish Science for Young Scientists
2002. Postdoctoral fellowship from the Foundation for Polish Science
2002. Prime Minister Award for PhD thesis
2005. Jakub Karol Parnas award for the best Polish experimental work in Biochemistry
2006. EMBO Installation Grant
2009. Scholarship from the Minister of Science and Higher Education for outstanding young scientists
2010. Prime Minister Award for habilitation thesis
2012. ERC starting grant
2009. Member of Young Scientists Academy, Polish Academy of Sciences
2013. National Science Centre award for outstanding scientific achievements
2013. Jakub Karol Parnas award for the best Polish experimental work in Biochemistry