The laboratory has significant experience in analysis of macromolecular assemblies by affinity purification and mass spectrometry. We have also recently implemented genome wide siRNA screening technology. Our approach allows us to identify novel genes involved in nucleic acids metabolism.
The major projects involving high throughputs methodologies are:
- Characterization of mycobacterial protein complexes as a part of large European consortium, SystemTB
- Proteomic analysis of macromolecular assemblies involved in RNA decay in vertebrates using the DT40 chicken cell line as a model system
- Identification of novel genes involved in RNA metabolism through genome wide siRNA screenings in mammalian cells
People involved:
Dominik Cysewski, Kamila Kłosowska, Anna Kotrys, Maria Macios, Kinga Orłowska, Patrycja Owczarek, Przemysław Płociński, Roman Szczęsny, Maciej Szewczyk
Selected publications:
A new strategy for gene targeting and functional proteomics using the DT40 cell line. Orlowska KP, Klosowska K, Szczesny RJ, Cysewski D, Krawczyk PS, Dziembowski A. Nucleic Acids Res. 2013 Sep;41(17):e167. doi: 10.1093/nar/gkt650. Epub 2013 Jul 27.
Strategies for the structural analysis of multi-protein complexes: lessons from the 3D-Repertoire project. Collinet B, Friberg A, Brooks MA, van den Elzen T, Henriot V, Dziembowski A, Graille M, Durand D, Leulliot N, Saint André C, Lazar N, Sattler M, Séraphin B, van Tilbeurgh H. J Struct Biol. 2011 Aug;175(2):147-58. doi: 10.1016/j.jsb.2011.03.018. Epub 2011 Apr 2.
Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations. Brooks MA, Gewartowski K, Mitsiki E, Létoquart J, Pache RA, Billier Y, Bertero M, Corréa M, Czarnocki-Cieciura M, Dadlez M, Henriot V, Lazar N, Delbos L, Lebert D, Piwowarski J, Rochaix P, Böttcher B, Serrano L, Séraphin B, van Tilbeurgh H, Aloy P, Perrakis A, Dziembowski A. Structure. 2010 Sep 8;18(9):1075-82.
